{"id":247,"date":"2012-11-05T16:42:14","date_gmt":"2012-11-05T20:42:14","guid":{"rendered":"https:\/\/sites.owu.edu\/wolverton\/?page_id=247"},"modified":"2016-12-01T08:50:41","modified_gmt":"2016-12-01T13:50:41","slug":"gene-expression","status":"publish","type":"page","link":"https:\/\/sites.owu.edu\/wolverton\/teaching\/cell-bio\/gene-expression\/","title":{"rendered":"Gene Expression"},"content":{"rendered":"<p><!--:en--><\/p>\n<h2 id=\"links\">Links<\/h2>\n<p><a href=\"http:\/\/www.nature.com\/principles\/ebooks\/introduction-to-cell-biology-4570805\/4570029\">Principles Module 50<\/a><\/p>\n<h2 id=\"objectives\">Objectives<\/h2>\n<ul>\n<li>Describe the design of the Beadle and Tatum experiment and how it provides evidence for the one gene, one polypeptide hypothesis<\/li>\n<li>Explain the central dogma<\/li>\n<\/ul>\n<h2 id=\"keypoints\">Key points<\/h2>\n<p>How are traits linked to inheritance?<\/p>\n<ul>\n<li>Beadle and Tatum used <em>Neurospora<\/em>\u00a0to study the relationship between DNA and traits<\/li>\n<li>they mutagenized spores and isolated colonies that couldn&#8217;t grow on minimal media (known as auxotrophic mutants)<\/li>\n<li>they transferred spores of auxotrophic mutants to arginine media and noted those that recovered, reasoning that they must be mutants in one of the steps of arginine biosynthesis<\/li>\n<li>they subcultured their mutants on media supplemented with an intermediate in the pathway and found classes of mutations that recovered:<\/li>\n<ul>\n<li>some recovered on media with ornithine, the first intermediate in the pathway<\/li>\n<li>some recovered on citruline, the second intermediate<\/li>\n<li>some only recovered on arginine, the product of the pathway<\/li>\n<\/ul>\n<li>their interpretation of their results was that each class of mutation corresponded to the loss of a specific enzyme in the pathway, and that by supplying the product of that enzyme they could rescue the growth on minimal media<\/li>\n<li>they concluded that one gene dictates the production of one enzyme, now revised to state that one gene dictates one polypeptide<\/li>\n<\/ul>\n<div>Central dogma<\/div>\n<div>\n<ul>\n<li>the idea that DNA encodes RNA, which in turn encodes proteins<\/li>\n<li>DNA is an information storing molecule, and the information it stores often is the instruction set for building a specific protein via messenger RNA (mRNA)<\/li>\n<li>DNA also encodes other forms of RNA that are catalytic or structural (ribosomal and transfer RNA)<\/li>\n<\/ul>\n<\/div>\n<h2 id=\"in-classactivities\">In-class activities<\/h2>\n<h2 id=\"questionsforpractice\">Questions for Practice<\/h2>\n<p><!--:--><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Links Principles Module 50 Objectives Describe the design of the Beadle and Tatum experiment and how it provides evidence for the one gene, one polypeptide hypothesis Explain the central dogma Key points How are traits linked to inheritance? Beadle and Tatum used Neurospora\u00a0to study the relationship between DNA and traits they mutagenized spores and isolated [&hellip;]<\/p>\n","protected":false},"author":40,"featured_media":0,"parent":447,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-247","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.owu.edu\/wolverton\/wp-json\/wp\/v2\/pages\/247","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.owu.edu\/wolverton\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.owu.edu\/wolverton\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.owu.edu\/wolverton\/wp-json\/wp\/v2\/users\/40"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.owu.edu\/wolverton\/wp-json\/wp\/v2\/comments?post=247"}],"version-history":[{"count":1,"href":"https:\/\/sites.owu.edu\/wolverton\/wp-json\/wp\/v2\/pages\/247\/revisions"}],"predecessor-version":[{"id":502,"href":"https:\/\/sites.owu.edu\/wolverton\/wp-json\/wp\/v2\/pages\/247\/revisions\/502"}],"up":[{"embeddable":true,"href":"https:\/\/sites.owu.edu\/wolverton\/wp-json\/wp\/v2\/pages\/447"}],"wp:attachment":[{"href":"https:\/\/sites.owu.edu\/wolverton\/wp-json\/wp\/v2\/media?parent=247"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}